MARC details
000 -LEADER |
fixed length control field |
03295nam a22004935i 4500 |
001 - CONTROL NUMBER |
control field |
978-3-030-94230-4 |
003 - CONTROL NUMBER IDENTIFIER |
control field |
DE-He213 |
005 - DATE AND TIME OF LATEST TRANSACTION |
control field |
20240729133942.0 |
007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION |
fixed length control field |
cr nn 008mamaa |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
fixed length control field |
220329s2022 sz | s |||| 0|eng d |
020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
ISBN |
9783030942304 |
-- |
978-3-030-94230-4 |
072 #7 - SUBJECT CATEGORY CODE |
Subject category code |
PSD |
Source |
bicssc |
072 #7 - SUBJECT CATEGORY CODE |
Subject category code |
SCI049000 |
Source |
bisacsh |
072 #7 - SUBJECT CATEGORY CODE |
Subject category code |
PSD |
Source |
thema |
100 1# - MAIN ENTRY--PERSONAL NAME |
Personal author |
Gautam, Akash. |
Relator term |
author. |
Relator code |
aut |
-- |
http://id.loc.gov/vocabulary/relators/aut |
245 10 - TITLE STATEMENT |
Title |
DNA and RNA Isolation Techniques for Non-Experts |
250 ## - EDITION |
Edition statement |
1st ed. 2022. |
264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
Place of production, publication, distribution, manufacture |
Cham : |
Name of producer, publisher, distributor, manufacturer |
Springer International Publishing : |
-- |
Imprint: Springer, |
Date of production, publication, distribution, manufacture, or copyright notice |
2022. |
300 ## - PHYSICAL DESCRIPTION |
Physical description |
XVI, 203 p. 58 illus., 48 illus. in color. |
Other physical details |
online resource. |
336 ## - CONTENT TYPE |
Content type term |
text |
Content type code |
txt |
Source |
rdacontent |
337 ## - MEDIA TYPE |
Media type term |
computer |
Media type code |
c |
Source |
rdamedia |
338 ## - CARRIER TYPE |
Carrier type term |
online resource |
Carrier type code |
cr |
Source |
rdacarrier |
490 1# - SERIES TITLE |
Series statement |
Techniques in Life Science and Biomedicine for the Non-Expert, |
International Standard Serial Number |
2367-1122 |
505 0# - CONTENTS |
Contents |
1-Basic Idea About Nucleic Acids For Non-Experts -- 2-General Steps During Isolation Of DNA And RNA -- 3-Phenol-Chloroform DNA Isolation Method -- 4-RNA Isolation By The Guanidinium-Acid-Phenol Method -- 5-Spin Column-Based Isolation Of Nucleic Acid -- 6-Isolation Of Plasmid DNA By Alkaline Lysis -- 7-DNA Isolation By Hydrophilic Ionic Liquid Treatment -- 8-Lithium Chloride Based Isolation Of DNA And RNA -- 9-Isolation Of Bacteriophage DNA By Peg Method -- 10-DNA Isolation By Chelex Method -- 11-DNA Isolation By Lysozyme And Proteinase K -- 12-Salting-Out Method For Blood Samples -- 13-CTAB Or SDA Based Isolation Of Plant's DNA -- 14-DNA Extraction By Spooling Method -- 15-Magnetic Bead-Based Nucleic Acid Isolation -- 16-Density Gradient-Based Nucleic Acid Isolation -- 17-DNA Extraction From Agarose Gel Though Paper-Strips -- 18-Transformation Or Genetic Modification Of Cells/Organisms -- 19-Gene Cloning And Vectors -- 20-Polymerase Chain Reaction (PCR) -- 21-Southern And Northern Blotting -- 22-Genome Mapping -- 23-Applications Of DNA Sequencing Technologies For Current Research. |
520 ## - ABSTRACT |
Abstract |
This thorough introductory volume presents the background, applications, and stepwise directions for standard DNA and RNA isolation techniques. Unlike a kit chemistry approach, this book provides a breadth of information necessary for junior or non-expert researchers to learn and apply these techniques in their work. An accessible, indispensable how-to guide for researchers in immunology, molecular biology, zoology, forensic science, genetics, botany, neuroscience, physiology, and others. |
650 #0 - SUBJECT HEADINGS |
Subject term |
Molecular biology. |
9 (RLIN) |
7018 |
650 #0 - SUBJECT HEADINGS |
Subject term |
Genetics. |
650 #0 - SUBJECT HEADINGS |
Subject term |
Cell nuclei. |
650 #0 - SUBJECT HEADINGS |
Subject term |
Cytology. |
650 14 - SUBJECT HEADINGS |
Subject term |
Molecular Biology. |
9 (RLIN) |
7018 |
650 24 - SUBJECT HEADINGS |
Subject term |
Genetics and Genomics. |
650 24 - SUBJECT HEADINGS |
Subject term |
Nuclear Organization. |
650 24 - SUBJECT HEADINGS |
Subject term |
Cell Biology. |
710 2# - ADDED CORPORATE NAME |
Added corporate author |
SpringerLink (Online service) |
830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE |
Uniform title |
Techniques in Life Science and Biomedicine for the Non-Expert, |
International Standard Serial Number |
2367-1122 |
856 ## - ONLINE RESOURCE |
Uniform Resource Identifier |
<a href="#gotoholdings">#gotoholdings</a> |
Link text |
Access resource |
245 ## - TITLE STATEMENT |
Medium |
[E-Book] |
347 ## - DIGITAL FILE CHARACTERISTICS |
File type |
text file |
Encoding format |
PDF |
Source |
rda |
912 ## - |
-- |
ZDB-2-SBL |
912 ## - |
-- |
ZDB-2-SXB |