MARC details
000 -LEADER |
fixed length control field |
03643nam a22005775i 4500 |
001 - CONTROL NUMBER |
control field |
978-3-031-19485-6 |
003 - CONTROL NUMBER IDENTIFIER |
control field |
DE-He213 |
005 - DATE AND TIME OF LATEST TRANSACTION |
control field |
20240729135904.0 |
007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION |
fixed length control field |
cr nn 008mamaa |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
fixed length control field |
230131s2023 sz | s |||| 0|eng d |
020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
ISBN |
9783031194856 |
-- |
978-3-031-19485-6 |
072 #7 - SUBJECT CATEGORY CODE |
Subject category code |
PSF |
Source |
bicssc |
072 #7 - SUBJECT CATEGORY CODE |
Subject category code |
PDN |
Source |
bicssc |
072 #7 - SUBJECT CATEGORY CODE |
Subject category code |
SCI008000 |
Source |
bisacsh |
072 #7 - SUBJECT CATEGORY CODE |
Subject category code |
PSF |
Source |
thema |
072 #7 - SUBJECT CATEGORY CODE |
Subject category code |
PDN |
Source |
thema |
100 1# - MAIN ENTRY--PERSONAL NAME |
Personal author |
Mani, Shalini. |
Relator term |
author. |
Relator code |
aut |
-- |
http://id.loc.gov/vocabulary/relators/aut |
245 10 - TITLE STATEMENT |
Title |
Animal Cell Culture: Principles and Practice |
250 ## - EDITION |
Edition statement |
1st ed. 2023. |
264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
Place of production, publication, distribution, manufacture |
Cham : |
Name of producer, publisher, distributor, manufacturer |
Springer International Publishing : |
-- |
Imprint: Springer, |
Date of production, publication, distribution, manufacture, or copyright notice |
2023. |
300 ## - PHYSICAL DESCRIPTION |
Physical description |
XVI, 327 p. 91 illus., 85 illus. in color. |
Other physical details |
online resource. |
336 ## - CONTENT TYPE |
Content type term |
text |
Content type code |
txt |
Source |
rdacontent |
337 ## - MEDIA TYPE |
Media type term |
computer |
Media type code |
c |
Source |
rdamedia |
338 ## - CARRIER TYPE |
Carrier type term |
online resource |
Carrier type code |
cr |
Source |
rdacarrier |
490 1# - SERIES TITLE |
Series statement |
Techniques in Life Science and Biomedicine for the Non-Expert, |
International Standard Serial Number |
2367-1122 |
505 0# - CONTENTS |
Contents |
1. Overview to animal cell culture -- 2. Cell culture laboratory -- 3. Good lab practices and bio-safety measures -- 4. Managing sterility in the lab -- 5. Media and buffer preparation for cell culture -- 6. Properties of cultured cells and selection of culture media -- 7. Selection and maintenance of cultured cells -- 8. Inoculation and passaging of adherent cells -- 9. Counting of cells -- 10. Cryopreservation of cell lines -- 11. Resuscitation of frozen cell line -- 12. Isolation and culturing of cells from different tissues -- 13. Differentiation, transformation and immortalization of cells -- 14. Co-culture techniques -- 15. 3d cell culture techniques -- 16. Stem cell culture techniques -- 17. Identification and removal of biological contamination in the media and cell suspensions -- 18. Analysis of cell growth kinetics in suspension and adherent types cell line -- 19. Applications of animal cell culture based assays -- 20. Ethical issues in animal cell culture -- 21. Common troubleshooting methods in cell culture techniques. |
520 ## - ABSTRACT |
Abstract |
This introductory guide provides novice researchers and lab students with a thorough step-by-step approach to standard animal cell culture techniques. Coverage includes lab safety and best practices, sterility management, preparation, ethical considerations, and troubleshooting for common pain points. This is an up-to-date, indispensable handbook for early-career researchers and students, as well as established scientists in biotechnology, cell and developmental biology, pharmaceutical toxicology, cytogenetics, and more. . |
650 #0 - SUBJECT HEADINGS |
Subject term |
Cytology |
General subdivision |
Technique. |
650 #0 - SUBJECT HEADINGS |
Subject term |
Microbiology |
General subdivision |
Technique. |
650 #0 - SUBJECT HEADINGS |
Subject term |
Biotechnology. |
9 (RLIN) |
5393 |
650 #0 - SUBJECT HEADINGS |
Subject term |
Cytology. |
650 #0 - SUBJECT HEADINGS |
Subject term |
Biology |
General subdivision |
Technique. |
650 #0 - SUBJECT HEADINGS |
Subject term |
Experimental immunology. |
650 14 - SUBJECT HEADINGS |
Subject term |
Cytological Techniques. |
650 24 - SUBJECT HEADINGS |
Subject term |
Microbiology Techniques. |
650 24 - SUBJECT HEADINGS |
Subject term |
Biotechnology. |
9 (RLIN) |
5393 |
650 24 - SUBJECT HEADINGS |
Subject term |
Cell Biology. |
650 24 - SUBJECT HEADINGS |
Subject term |
Immunological Techniques. |
700 1# - ADDED PERSONAL NAME |
Added personal author |
Singh, Manisha. |
Relator term |
author. |
700 1# - ADDED PERSONAL NAME |
Added personal author |
Kumar, Anil. |
Relator term |
author. |
710 2# - ADDED CORPORATE NAME |
Added corporate author |
SpringerLink (Online service) |
830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE |
Uniform title |
Techniques in Life Science and Biomedicine for the Non-Expert, |
International Standard Serial Number |
2367-1122 |
856 ## - ONLINE RESOURCE |
Uniform Resource Identifier |
<a href="#gotoholdings">#gotoholdings</a> |
Link text |
Access resource |
245 ## - TITLE STATEMENT |
Medium |
[E-Book] |
347 ## - DIGITAL FILE CHARACTERISTICS |
File type |
text file |
Encoding format |
PDF |
Source |
rda |
912 ## - |
-- |
ZDB-2-SBL |
912 ## - |
-- |
ZDB-2-SXB |