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Manual of research techniques in cardiovascular medicine (Record no. 57428)

MARC details
000 -LEADER
fixed length control field 03840cam a2200325 4500
001 - CONTROL NUMBER
control field 9781118495162
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 180406t2014 xxu||||| |||| 00| 0 eng d
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number 9781118495162
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number 1118495160 (electronic bk.)
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number 9781118495148 (electronic bk.)
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number 1118495144 (electronic bk.)
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number 9781118495131 (electronic bk.)
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number 1118495136 (electronic bk.)
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number 9781306219426
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number 1306219426
100 ## - MAIN ENTRY--PERSONAL NAME
Personal name Ardehali, Hossein
245 #0 - TITLE STATEMENT
Title Manual of research techniques in cardiovascular medicine
Medium [electronic resource]
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc. Chichester, West Sussex; Hoboken :
Name of publisher, distributor, etc. Wiley,
Date of publication, distribution, etc. 2014
300 ## - PHYSICAL DESCRIPTION
Extent 1 online resource (473 pages) : illustrations : (electronic bk.)
505 ## - FORMATTED CONTENTS NOTE
Formatted contents note text
505 ## - FORMATTED CONTENTS NOTE
Formatted contents note Cover; Dedication; Title page; Copyright page; Contents; List of Contributors; Preface; About the Companion Website; Part 1: Electrophysiology; 1: Measurement of calcium transient ex vivo; Introduction; Preparation and fluorescent indicator loading; Optical setup; Calibration; Analysis and interpretation; Set-by-step procedure; References; 2: Confocal imaging of intracellular calcium cycling in isolated cardiac myocytes; Introduction; Materials and methods; Chamber, electrical stimulation, and superfusate delivery apparatus; Confocal microscope system.
505 ## - FORMATTED CONTENTS NOTE
Formatted contents note Cell loading of fluorescent Ca2+ indicatorsExperimental approach; Data analyses; Weaknesses and strengths of the method and alternative approaches; Conclusions; References; 3: Generating a large animal model of persistent atrial fibrillation; Introduction; Insights from tachypacing-induced AF models; Protocol; Generating a tachypacing-induced AF model; Pacing protocols; Ventricular rate during AF: to ablate or not to ablate; Strengths and weaknesses of the model; Complications and alternative approaches to study AF; Potential complications; Alternative AF models; Conclusion; Acknowledgement.
505 ## - FORMATTED CONTENTS NOTE
Formatted contents note Choices to make before an experimentAP recording procedure; Frequent problems; AP analysis; Alternative approaches and extensions; Weaknesses and strengths; Conclusion; References; 6: Patch-clamp recordings from isolated cardiac myocytes; Introduction; Theory of patch-clamp recording; Cell-attached configuration; Whole-cell recording configuration; Inside-out excised patch configuration; Outside-out excised patch configuration; Instrumentation for patch-clamp recordings; Experimental aspects of patch-clamp recordings; Gigaseal formation; Whole-cell recordings.
505 ## - FORMATTED CONTENTS NOTE
Formatted contents note Whole-cell voltage-clamp protocols, data acquisition, and analysisConclusions and future directions; References; 7: Optical mapping of the heart; Introduction; Protocols; Experimental set-up; Tissue preparations (Figure 7.2); Staining and image acquisition; Data processing and analysis; Representative results; Alternative approaches; Weaknesses and strengths of method; Conclusions; References; Part 2: Isolation and Maintenance of Primary Stem Cells; 8: Isolation of colony-forming endothelial progenitor cells; Introduction; Materials; Laboratory reagents; Laboratory equipment; Methods."
520 ## - SUMMARY, ETC.
Summary, etc. Includes bibliographical references and index.
520 ## - SUMMARY, ETC.
Summary, etc. While some research methods or techniques are applicable in several areas of medicine, research in cardiovascular diseases requires knowledge of an increasing array of procedures, techniques and measurements that are highly specialized and unique to this area of investigation. Edited by senior clinical investigators who are recognized leaders in cardiovascular medicine worldwide, this book provides readers with a comprehensive, practical ""how-to-do-it"" review of best-practice techniques for cardiovascular research.
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Bolli, Roberto
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Losordo, Douglas
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="#gotoholdings">#gotoholdings</a>
Link text Access resource
Holdings
Withdrawn status Lost status Damaged status Not for loan Home library Current library Shelving location Date acquired Total Checkouts Full call number Date last seen Uniform Resource Identifier Price effective from Koha item type Public note
        Newham Library (Barts Health) Newham Library (Barts Health) Online 03/10/2023     03/10/2023 http://dx.doi.org/10.1002/9781118495148 03/10/2023 Electronic book [Wiley Online Library]
        Royal London Library (Barts Health) Royal London Library (Barts Health) Online 03/10/2023     03/10/2023 http://dx.doi.org/10.1002/9781118495148 03/10/2023 Electronic book [Wiley Online Library]
        St Bartholomew's Library (Barts Health) St Bartholomew's Library (Barts Health) Online 20/04/2022   WG 120 20/04/2022 http://dx.doi.org/10.1002/9781118495148 20/04/2022 Electronic book [Wiley Online Library]
        Whipps Cross Library (Barts Health) Whipps Cross Library (Barts Health) Online 03/10/2023     03/10/2023 http://dx.doi.org/10.1002/9781118495148 03/10/2023 Electronic book [Wiley Online Library]
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