TY  - BOOK
AU  - Mani,Shalini
AU  - Singh,Manisha
AU  - Kumar,Anil
ED  - SpringerLink (Online service)
TI  - Animal Cell Culture: Principles and Practice
T2  - Techniques in Life Science and Biomedicine for the Non-Expert,
SN  - 9783031194856
PY  - 2023///
CY  - Cham
PB  - Springer International Publishing, Imprint: Springer
KW  - Cytology
KW  - Technique
KW  - Microbiology
KW  - Biotechnology
KW  - Biology
KW  - Experimental immunology
KW  - Cytological Techniques
KW  - Microbiology Techniques
KW  - Cell Biology
KW  - Immunological Techniques
N1  - 1. Overview to animal cell culture -- 2. Cell culture laboratory -- 3. Good lab practices and bio-safety measures -- 4. Managing sterility in the lab -- 5. Media and buffer preparation for cell culture -- 6. Properties of cultured cells and selection of culture media -- 7. Selection and maintenance of cultured cells -- 8. Inoculation and passaging of adherent cells -- 9. Counting of cells -- 10. Cryopreservation of cell lines -- 11. Resuscitation of frozen cell line -- 12. Isolation and culturing of cells from different tissues -- 13. Differentiation, transformation and immortalization of cells -- 14. Co-culture techniques -- 15. 3d cell culture techniques -- 16. Stem cell culture techniques -- 17. Identification and removal of biological contamination in the media and cell suspensions -- 18. Analysis of cell growth kinetics in suspension and adherent types cell line -- 19. Applications of animal cell culture based assays -- 20. Ethical issues in animal cell culture -- 21. Common troubleshooting methods in cell culture techniques
N2  - This introductory guide provides novice researchers and lab students with a thorough step-by-step approach to standard animal cell culture techniques. Coverage includes lab safety and best practices, sterility management, preparation, ethical considerations, and troubleshooting for common pain points. This is an up-to-date, indispensable handbook for early-career researchers and students, as well as established scientists in biotechnology, cell and developmental biology, pharmaceutical toxicology, cytogenetics, and more.
UR  - #gotoholdings
ER  -