Histological and Histochemical Methods: Theory and Practice

Monograph

The chemical and physical principles of fixation, staining, and histochemistry in one complete volume! Now in its fourth edition, "Histological and Histochemical Methods" has been expanded and thoroughly updated with the latest techniques and developments within the field, whilst retaining the details of the classic techniques still in use. The relations of chemical structures and reactions to fixation, tissue processing, staining, enzyme location, immunohistochemistry and other procedures are explained in simple, descriptive terms. As well as being a textbook, further references and more detailed information are available for those who wish to study a topic in more depth. This book is firmly established as a resource for researchers, students and technologists in biological and biomedical sciences for more than 25 years. There are comprehensive discussions of the chemical and biological mechanisms. Understanding of these mechanisms is a prerequisite for troubleshooting, and for the detailed practical instructions for using the techniques which follow. The integration of the theoretical and practical material enables the reader to understand the reasoning behind each step. Anyone preparing biological specimens for light microscopy will find this to be an invaluable companion. This book is recommended for lecturers and students in biological and biomedical disciplines using histological and histochemical techniques. Postgraduate researchers will also find this to be the ideal laboratory companion, as will medical laboratory technologists preparing for their professional examinations.