000 03643nam a22005775i 4500
001 978-3-031-19485-6
003 DE-He213
005 20240729135904.0
007 cr nn 008mamaa
008 230131s2023 sz | s |||| 0|eng d
020 _a9783031194856
_9978-3-031-19485-6
024 7 _a10.1007/978-3-031-19485-6
_2doi
072 7 _aPSF
_2bicssc
072 7 _aPDN
_2bicssc
072 7 _aSCI008000
_2bisacsh
072 7 _aPSF
_2thema
072 7 _aPDN
_2thema
100 1 _aMani, Shalini.
_eauthor.
_4aut
_4http://id.loc.gov/vocabulary/relators/aut
245 1 0 _aAnimal Cell Culture: Principles and Practice
250 _a1st ed. 2023.
264 1 _aCham :
_bSpringer International Publishing :
_bImprint: Springer,
_c2023.
300 _aXVI, 327 p. 91 illus., 85 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aTechniques in Life Science and Biomedicine for the Non-Expert,
_x2367-1122
505 0 _a1. Overview to animal cell culture -- 2. Cell culture laboratory -- 3. Good lab practices and bio-safety measures -- 4. Managing sterility in the lab -- 5. Media and buffer preparation for cell culture -- 6. Properties of cultured cells and selection of culture media -- 7. Selection and maintenance of cultured cells -- 8. Inoculation and passaging of adherent cells -- 9. Counting of cells -- 10. Cryopreservation of cell lines -- 11. Resuscitation of frozen cell line -- 12. Isolation and culturing of cells from different tissues -- 13. Differentiation, transformation and immortalization of cells -- 14. Co-culture techniques -- 15. 3d cell culture techniques -- 16. Stem cell culture techniques -- 17. Identification and removal of biological contamination in the media and cell suspensions -- 18. Analysis of cell growth kinetics in suspension and adherent types cell line -- 19. Applications of animal cell culture based assays -- 20. Ethical issues in animal cell culture -- 21. Common troubleshooting methods in cell culture techniques.
520 _aThis introductory guide provides novice researchers and lab students with a thorough step-by-step approach to standard animal cell culture techniques. Coverage includes lab safety and best practices, sterility management, preparation, ethical considerations, and troubleshooting for common pain points. This is an up-to-date, indispensable handbook for early-career researchers and students, as well as established scientists in biotechnology, cell and developmental biology, pharmaceutical toxicology, cytogenetics, and more. .
650 0 _aCytology
_xTechnique.
650 0 _aMicrobiology
_xTechnique.
650 0 _aBiotechnology.
_95393
650 0 _aCytology.
650 0 _aBiology
_xTechnique.
650 0 _aExperimental immunology.
650 1 4 _aCytological Techniques.
650 2 4 _aMicrobiology Techniques.
650 2 4 _aBiotechnology.
_95393
650 2 4 _aCell Biology.
650 2 4 _aImmunological Techniques.
700 1 _aSingh, Manisha.
_eauthor.
_4aut
_4http://id.loc.gov/vocabulary/relators/aut
700 1 _aKumar, Anil.
_eauthor.
_4aut
_4http://id.loc.gov/vocabulary/relators/aut
710 2 _aSpringerLink (Online service)
830 0 _aTechniques in Life Science and Biomedicine for the Non-Expert,
_x2367-1122
856 _u#gotoholdings
_yAccess resource
912 _aZDB-2-SBL
912 _aZDB-2-SXB
245 _h[E-Book]
999 _c103362
_d103362